膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Hybridoma Monoclonal
种属反应性（Reactivity）
Chemical
免疫原（Immunogen）
DM-1.
特异性（Specificity）
This product is a specific antibody specifically reacts with DM-1&DM-4.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.4-200 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (Cat. No. DM1-Y73) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (Cat. No. DM1-Y73) at 2 μg/mL, add increasing concentrations of Trastuzumab-DM1 (T-DM1), and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 8 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.5 ng/mL (QC tested).
Protocol
Immobilized ADC-DM4 at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (Cat. No. DM1-Y73) with a linear range of 0.20-6.25ng/mL (Routinely tested).
Protocol
Immobilized Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (Cat. No. DM1-Y73) at 5 μg/mL, add Trastuzumab-DM1 in the 50% Human serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
背景（Background）
Mertansine (DM-1) is a tubulin inhibitor that binds to the ends of microtubules and inhibits microtubule dynamics. DM-1(Mertansine) has antitumor activity and functions as a regulator of tubulin. It is an alpha-amino acid ester, a carbamate, an epoxide, an organic heterocyclic tetracyclic compound, an organochlorine compound, a mercaptan, and a maydenin alkaloid. DM-1, derived from Mydenin, is a cytotoxic component of antibody-drug conjugations that produce antibody-drug conjugations via a sulfhydryl group splice with SPP (n-succinimide 4- (2-pyridyl dithio)) or SMCC (4- (3-mercapto-2, 5-dioxy-1 pyrrolidyl) -cyclohexanic acid) splice.
