分子别名(Synonym)
Cadherin-17,CDH17,HPT-1,LI-cadherin
表达区间及表达系统(Source)
Biotinylated Mouse Cadherin-17 Protein, His,Avitag (CA7-M82E7) is expressed from human 293 cells (HEK293). It contains AA Phe 26 - Met 786 (Accession # Q9R100-1).
Predicted N-terminus: Phe 26
Request for sequence
蛋白结构(Molecular Characterization)
![Cadherin-17 Structure](/images/structure_Images/CA7-M82E7-STC2.jpg)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 87.9 kDa. The protein migrates as 110-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
![Cadherin-17 SDS-PAGE](images/sds/CA7-M82E7.jpg)
Biotinylated Mouse Cadherin-17 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
![Cadherin-17 SEC-MALS](images/mals/CA7-M82E7-M1.jpg)
The purity of Biotinylated Mouse Cadherin-17 Protein, His,Avitag (Cat. No. CA7-M82E7) is more than 95% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
![Cadherin-17 ELISA](images/elisa/CA7-M82E7-E1.jpg)
Immobilized Biotinylated Mouse Cadherin-17 Protein, His,Avitag (Cat. No. CA7-M82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of Rabbit Anti-Cadherin 17 (QC tested).
Protocol
背景(Background)
Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.