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Biotinylated Human EphA2 Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

EphA2

表达区间及表达系统(Source)

Biotinylated Human EphA2 Protein, His,Avitag (EP2-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Asn 534 (Accession # P29317).

Predicted N-terminus: Ala 24

Request for sequence

蛋白结构(Molecular Characterization)

EphA2 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 59.8 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

EphA2 SDS-PAGE

Biotinylated Human EphA2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

EphA2 SEC-MALS

The purity of Biotinylated Human EphA2 Protein, His,Avitag (Cat. No. EP2-H82E4) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

EphA2 ELISA

Immobilized Biotinylated Human EphA2 Protein, His,Avitag (Cat. No. EP2-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Ephrin-A1, Fc Tag (Cat. No. EF1-H5251) with a linear range of 0.1-1 ng/mL (QC tested).

Protocol

 

背景(Background)

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EF1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EF5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EF2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

 

前沿进展

 
 
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EphA2靶点信息
英文全称:Ephrin type-A receptor 2
中文全称:A型肝配蛋白受体-2
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:4详情
最高研发阶段:批准上市
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