登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Syndecan-1 >SY1-HA2H9

APC-Labeled Human Syndecan-1 Protein, His Tag (Site-specific conjugation)

分子别名(Synonym)

SDC1,Syndecan-1,CD138,SYND1,SDC

表达区间及表达系统(Source)

APC-Labeled Human Syndecan-1 Protein, His Tag (SY1-HA2H9) is produced via conjugation of APC to Human Syndecan-1 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Syndecan-1 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).

Predicted N-terminus: Gln 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 27.5 kDa.

偶联(Conjugate)

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

应用说明(Application)

Please note that this product is NOT compatible to streptavidin detection system.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

活性(Bioactivity)-FACS

Syndecan-1 FACS

5e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human Syndecan-1 Protein, His Tag (Cat. No. SY1-HA2H9) and negative control protein respectively. APC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Syndecan-1靶点信息
英文全称:Syndecan-1
中文全称:多配体蛋白聚糖-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细
相关产品
View All Syndecan-1

消息提示

请输入您的联系方式,再点击提交!

确定