膜杰作
Star Staining
| ID | Components | Size |
| RAS168-C01 | Pre-coated RSV-Post-Fusion glycoprotein F0 Microplate | 1 plate |
| RAS168-C02 | RSV-F0 Antibody Positive Control | 100 μL |
| RAS168-C03 | RSV-F0 Antibody Negative Control | 100 μL |
| RAS168-C04 | HRP-Conjugated Antibody | 50 μL |
| RAS168-C05 | 10 x Washing Buffer | 50 mL |
| RAS168-C06 | Dilution Buffer | 50 mL |
| RAS168-C07 | Substrate Solution | 12 mL |
| RAS168-C08 | Stop Solution | 7 mL |
背景（Background）
Most in vitro RSV neutralizing antibodies in human sera are directed against the prefusion conformation, but due to its instability the prefusion conformation has a propensity to prematurely refold into the stable postfusion conformation, both in solution and on the surface of the virions.
An RSV F protein that has both high expression levels and maintains a stable prefusion conformation would, therefore, be a promising subunit vaccine candidate against RSV.
To facilitate the RSV-related research, drug trials and vaccine development,a high-throughput assay to measure IgG antibodies against the virus is in urgent need.
应用说明（Application）
The kit is developed for titer measurement of Anti-RSV-F0 Antibody IgG (Post-Fusion glycoprotein F0) in Mouse serum.
It is for research use only.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-RSV-Post-Fusion glycoprotein F0 antibodies in Mouse serum by Post-Fusion glycoprotein F0. The Kit consists of Pre-coated RSV-Post-Fusion glycoprotein F0 Microplate,Positive Control,Negative Control and HRP-Conjugated antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
