膜杰作
Star Staining
描述（Description）
The Human NKp46 (Luc) Jurkat Reporter Cell was engineered to not only express the NFAT response element driving luciferase expressing systems, but also express the receptor full length human NKp46 (Gene ID: 9437). When cocultured with anti-human Nkp46 agonist antibody, the interaction of agonist antibody and NKp46 on the surface of Human NKp46 (Luc) Jurkat Reporter Cell results in NFAT-mediated luminescence.
应用说明（Application）
Screen for anti-human NKp46 agonist antibody.
生长特性（Growth Properties）
Suspension
筛选标记（Selection Marker）
Puromycin (5 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
RPMI-1640 + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human NKp46 on Human NKp46 (Luc) Jurkat Reporter Cell by FACS.
Cell surface staining was performed on Human NKp46 (Luc) Jurkat Reporter Cell or negative control cell using APC- labeled Anti-human NKp46 antibody.
Protocol
Application
Agonistic activity analysis of anti-human NKp46 antibody (RLU).
This reporter cell was incubated with serial dilutions of anti-human NKp46 antibody. The EC50 of anti-human NKp46 antibody was approximately 0.1011 μg/mL.
Protocol
Agonistic activity analysis of anti-human NKp46 antibody (FOLD).
This reporter cell was incubated with serial dilutions of anti-human NKp46 antibody. The max induction fold was approximately 20.61.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human NKp46 (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of anti-human NKp46 antibody. Anti-human NKp46 antibody stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 6-21.
Protocol
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背景（Background）
Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1 contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
