膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The HEK293/Human Integrin alpha V beta 6 Stable Cell Line was engineered to express the receptors full length human Integrin alpha V (Gene ID: 3685) and Integrin beta 6 (Gene ID: 3694), used to mimic cancer target cells. Surface expression of human Integrin alpha V beta 6 was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based Integrin alpha V beta 6 binding assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human Integrin alpha V on HEK293/Human Integrin alpha V beta 6 Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human Integrin alpha V beta 6 Stable Cell Line or negative control cell using anti-human Integrin alpha V antibody followed by staining with FITC anti-mouse IgG Antibody.
Protocol
Expression analysis of human Integrin beta 6 on HEK293/Human Integrin alpha V beta 6 Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human Integrin alpha V beta 6 Stable Cell Line or negative control cell using anti-human Integrin beta 6 antibody followed by staining with PE anti-human IgG Fc Antibody.
Protocol
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背景（Background）
Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.
Limited Use&License Disclosure
- This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
