膜杰作
Star Staining
描述（Description）
The HEK293/Human Cadherin-6 Stable Cell Line was engineered to express the receptor full length human Cadherin-6 (Gene ID: 414842), used to mimic cancer target cells. Surface expression of Human Cadherin-6 was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based Cadherin-6 binding assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human Cadherin-6 on HEK293/Human Cadherin-6 Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human Cadherin-6 Stable Cell Line or negative control cell using anti-human Cadherin-6 antibody followed by staining with PE anti-human IgG Fc antibody.
Protocol
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背景（Background）
Cadherin-6 (CDH6) is also known as Kidney cadherin (K-cadherin or KCAD), is a a type II classical cadherin from the cadherin superfamily. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH6 / KCAD contains five cadherin domains. CDH6 is highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression and also expressed in certain liver and kidney carcinomas.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
