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ClinMax™ Human MCP-1 / CCL2 ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human MCP-1/CCL2 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human CCL2 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human CCL2 in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

CCL2 Workflow

关键信息(Key Features)

AnalyteMCP-1
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity2pg/mL
Range4.688 pg/mL-300 pg/mL
Assay Time3hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

CCL2 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of MCP-1 is less than 2 pg/mL.

 

验证(Validation)

回收率(Recovery)

Recombinant MCP-1 was spiked into 3 human serum samples, and then analyzed. The average recovery of MCP-1 for serum samples is 108%.

CCL2 RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CEA036-C01Pre-coated Anti-MCP-1 Antibody Microplate1 plate
CEA036-C02Human MCP-1 Standard150 ng×2
CEA036-C03Biotin-Anti-MCP-1 Antibody Con. Solution150 μL
CEA036-C04Biotin-Antibody Dilution Buffer8 mL
CEA036-C05Streptavidin-HRP Con. Solution500 μL
CEA036-C06Streptavidin-HRP Dilution Buffer15 mL
CEA036-C0720× Washing Buffer50 mL
CEA036-C08Sample Dilution Buffer15 mL ×2
CEA036-C09Substrate Solution12 mL
CEA036-C10Stop Solution6 mL

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
CCL2靶点信息
英文全称:C-C motif chemokine 2
中文全称:C-C基序趋化因子-2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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