登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Tyk2 >TY2-H5547

Human Tyk2 Protein, His Tag (active enzyme)

分子别名(Synonym)

Tyk2,tyrosine kinase 2

表达区间及表达系统(Source)

Human Tyk2 Protein, His Tag (TY2-H5547) is expressed from Baculovirus-Insect cells. It contains AA Pro 871 - Cys 1187 (Accession # P29597-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

Tyk2 Structure

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 38.5 kDa. The protein migrates as 53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

活性(Bioactivity)

The Tyk2 assay is performed using the ADP-GloTM Kinase Assay kit which quantifies the amount of ADP produced by the Tyk2 reaction. The ADP- GloTM Reagent is added to terminate the kinase reaction and to deplete the remaining ATP, and then the Kinase Detection Reagent is added to convert ADP to ATP and to measure the newly synthesized ATP using luciferase/luciferin reaction.The specific activity is >400 pmol/min/mg (QC tested).

Protocol

 

背景(Background)

Tyk2 was the first member of the JAK family that was described (the other members are JAK1, JAK2, and JAK3). It has been implicated in IFN-α, IL-6, IL-10 and IL-12 signaling. Tyk2 functions primarily in IL-12 and type I-IFN signaling. Tyk2 deficiency has more dramatic effects in human cells than in mouse cells. However, in addition to IFN-α and -β and IL-12 signaling, Tyk2 has major effects on the transduction of IL-23, IL-10, and IL-6 signals. Recently, it has been recognized that IL-12 and IL-23 share ligand and receptor subunits that activate Tyk2. Tyk2 is activated by IL-10, and its deficiency affects the ability to generate and respond to IL-10.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Tyk2靶点信息
英文全称:Tyrosine-protein kinase 2
中文全称:酪氨酸蛋白激酶TYK2
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:8详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细
相关产品
View All Tyk2

消息提示

请输入您的联系方式,再点击提交!

确定