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 >  Protein>Transferrin >TRN-H52H3

Human Transferrin Protein, His Tag (MALS verified)

分子别名(Synonym)

Transferrin,TF,DKFZp781D0156,PRO1557,PRO2086

表达区间及表达系统(Source)

Human Transferrin Protein, His Tag (TRN-H52H3) is expressed from human 293 cells (HEK293).

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 77.1 kDa. The protein migrates as 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

Transferrin SDS-PAGE

Human Transferrin Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

Transferrin SEC-MALS

The purity of Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3) is more than 95% and the molecular weight of this protein is around 70-95 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Transferrin ELISA

Immobilized Biotinylated Human Transferrin R Protein, Fc,Avitag (Cat. No. TFR-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3)) with a linear range of 5-78 ng/mL (QC tested).

Protocol

Transferrin ELISA

Immobilized Human Transferrin Protein, His Tag (Cat. No. TRN-H52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R Protein, Fc,Avitag (Cat. No. TFR-H82F3) with a linear range of 5-78 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Transferrin is also known as Serotransferrin, Beta-1 metal-binding globulin, TF, and is iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). The affinity of transferrin for Fe(III) is extremely high (1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.When not bound to iron, it is known as "apo-transferrin”. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains (the first situated in the N-terminus and the second in the C-terminus). The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. The liver is the main source of manufacturing transferrin, but other sources such as the brain also produce this molecule . Transferrin is also associated with the innate immune system. Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron that impedes bacteria survival in a process called iron withholding. The level of transferrin decreases in inflammation. The metal binding properties of transferrin have a great influence on the biochemistry of plutonium in humans. Transferrin has a bacteriocidal effect on bacteria, in that it makes Fe3+ unavailable to the bacteria.Carbohydrate deficient transferrin increases in the blood with heavy ethanol consumption and can be monitored via laboratory testing.

 

前沿进展

 
 
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Transferrin靶点信息
英文全称:Tissue factor
中文全称:组织因子
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:4详情
最高研发阶段:批准上市
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