膜杰作
Star Staining
分子别名（Synonym）
EphA2
表达区间及表达系统（Source）
Rat EphA2 Protein, His Tag (EP2-R52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Met 536 (Accession # D3ZBN3).
Predicted N-terminus: Gln 24
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 58.6 kDa. The protein migrates as 60-67 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rat EphA2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rat EphA2 Protein, His Tag (Cat. No. EP2-R52H5) is more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Ephrin-A1, Fc Tag (Cat. No. EF1-H5251) at 5 μg/mL (100 μL/well) can bind Rat EphA2 Protein, His Tag (Cat. No. EP2-R52H5) with a linear range of 0.01-0.156 (QC tested).
Protocol
Immobilized Rat EphA2 Protein, His Tag (Cat. No. EP2-R52H5) at 5 μg/mL (100 μL/well) can bind Human Ephrin-A1, Fc Tag (Cat. No. EF1-H5251) with a linear range of 0.04-1 ng/mL (Routinely tested).
Protocol
背景（Background）
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EF1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EF5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EF2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
