膜杰作
Star Staining
分子别名（Synonym）
KIAA1754L
表达区间及表达系统（Source）
Human ITPRIPL1 Protein, His Tag (IT1-H52H3) is expressed from human 293 cells (HEK293). It contains AA His 25 - Gly 103 (Accession # Q6GPH6).
Predicted N-terminus: His 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 11.2 kDa. The protein migrates as 11 kDa and 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human ITPRIPL1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human ITPRIPL1 Protein, His Tag (Cat. No. IT1-H52H3) at 1 μg/mL (100 μL/well) can bind ITPRIPL1 antibody with a linear range of 0.01-1.25 μg/mL (QC tested).
Protocol
背景（Background）
Inositol 1,4,5-triphosphate receptor interacting protein like 1 (ITPRIPL1) is a single transmembrane protein，exhibits normal testis-enriched expression and widespread overexpression in various human cancers. ITPRIPL1 is commonly found in tumors with low PD-L1 expression. ITPRIPL1 acts as a natural ligand for CD3 ε, evading immune surveillance, leading to decreased T cell activity and promoting tumor growth. Research has shown that inducing stable CD3E-NCK1 binding prevents CD3E-ZAP70 interaction, subsequently inhibiting downstream ERK-NFkB signaling cascade and calcium influx activation.
