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Varicella Zoster Virus Glycoprotein H&L (gH&gL) ELISA Kit

For research use only.

组分(Materials Provided)

IDComponentsSize
RAS222-C01Pre-coated Anti-Glycoprotein H&L (VZV) Antibody Microplate1 plate
RAS222-C02Glycoprotein H&L (VZV) Standard20 μg
RAS222-C03HRP-Anti-Glycoprotein H&L (VZV) Antibody20 μg
RAS222-C0410×Washing Buffer 50 mL
RAS222-C052×Dilution Buffer50 mL
RAS222-C06Substrate Solution12 mL
RAS222-C07Stop Solution7 mL

产品概述(Product Overview)

Varicella Zoster Virus Glycoprotein H&L (gH&gL) ELISA Kit is based on the sandwich-ELISA method and is used to detect and quantitatively determine the levels of VZV Glycoprotein H&L (gH&gL) in vaccine samples. The kit is designed to provide a reliable solution for the manufacturing and quality control of VZV vaccine development.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储(Storage)

2-8℃

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

Glycoprotein H & Glycoprotein L/gH & gL (VZV) TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

 
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前沿进展

Induction of cellular immunity to varicella-zoster virus glycoproteins tested with pernasal coadministration of Escherichia coli enterotoxin in mice
Tsuji, Shiraki, Sato et al
J Med Virol (2003) 69 (3), 451-8
Abstract: A mutant of Escherichia coli enterotoxin promotes the induction of cellular immunity to a live varicella vaccine (the Oka strain) as a mucosal adjuvant in mice. An investigation was carried out to determine which of the purified glycoproteins of the virus among three induced cellular immunity with a single nasal administration. Spleen cells from mice immunized nasally with the vaccine and toxin produced interleukin-2 (IL-2) at the same level on restimulation in vitro with glycoprotein H: glycoprotein L (gH:gL), gB, and gE:gI, but not IL-4. The spleen cells from mice immunized with gH:gL, gB, or gE:gI and toxin produced IL-2 on restimulation with gH:gL, gB, or gE:gI, respectively, and the vaccine, but not IL-4. Immunization with gH:gL and the toxin showed increased thymidine uptake and production of IL-2 and interferon-gamma (IFN-gamma) of the spleen cells, but not IL-4, depending on the dose of gH:gL used for immunization and restimulation in vitro. Purified gE:gI and gB have been reported to be the strongest stimulators of cellular immunity to varicella upon subcutaneous injection and are useful as a subunit vaccine. All the glycoproteins tested are excellent stimulators of cellular immunity to the virus and itself on nasal co-immunization with the toxin.Copyright 2003 Wiley-Liss, Inc.
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