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 >  Protein>Flt-3 Ligand >FLL-H5218

Human Flt-3 Ligand Protein, premium grade

GMP version GMP-FLLH28 is now available for seamless transition.

分子别名(Synonym)

FLT3LG,FL,FLT3L,Flt3 ligand

表达区间及表达系统(Source)

Human Flt-3 Ligand, premium grade (FLL-H5218) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1).

Predicted N-terminus: Thr 27

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Request for sequence

蛋白结构(Molecular Characterization)

Flt-3 Ligand Structure

This protein carries no "tag".

The protein has a calculated MW of 18.0 kDa. The protein migrates as 24 kDa, 27 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

宿主蛋白残留(Host Cell Protein)

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留(Host Cell DNA)

<0.02 ng/μg of protein tested by qPCR.

无菌(Sterility)

Negative

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Flt-3 Ligand SDS-PAGE

Human Flt-3 Ligand, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

Flt-3 Ligand SEC-MALS

The purity of Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) is more than 95% and the molecular weight of this protein is around 42-52 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-Bioactivity CELL BASE

Flt-3 Ligand CELL

Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) stimulates proliferation of OCI-AML5 cell. The specific activity of Human Flt-3 Ligand, premium grade is > 5.00×10^5 IU/mg, which is calibrated against human WHO-Flt3 Ligand International Standard (NIBSC code: 96/532) (QC tested).

Protocol

 

活性(Bioactivity)-ELISA

Flt-3 Ligand ELISA

Immobilized Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) at 1 μg/mL (100 μL/well) can bind Human Flt-3, Fc Tag (Cat. No. FL3-H5258) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

 

稳定性(Stability)

Flt-3 Ligand STABILITY

The Cell based assay shows that Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) is stable at 37°C for 48 hours.

Flt-3 Ligand STABILITY

The Cell based assay shows that Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) is stable after freezing and thawing 3 times.

Flt-3 Ligand STABILITY

The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG Flt-3 Ligand.

 

背景(Background)

FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

 

前沿进展

 
 
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Flt-3 Ligand靶点信息
英文全称:Fms-related tyrosine kinase 3 ligand
中文全称:Flt3配体
种类:
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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