图1 利用磁珠进行抗体筛选的过程
分子 | 货号 | 产品描述 | 订购/预购 |
---|---|---|---|
ACE2 | MBS-K013 | Human ACE2-coupled Magnetic Beads | |
Spike RBD | MBS-K014 | Human Anti-SARS-CoV-2 Spike RBD Antibody-coupled Magnetic Beads | |
Spike S1 | MBS-K001 | SARS-CoV-2 Spike S1-coupled Magnetic Beads | |
Spike S1 | MBS-K002 | SARS-CoV-2 Spike RBD-coupled Magnetic Beads | |
Spike protein | MBS-K015 | SARS-CoV-2 Spike Trimer-coupled Magnetic Beads | |
Spike protein | MBS-K031 | SARS-CoV-2 Spike Trimer (P.1) Coupled Magnetic Beads | |
VEGF165 | MBS-K036 | Human VEGF165-coupled Magnetic Beads | |
CD19 | MBS-K005 | Human CD19-coupled Magnetic Beads | |
CD3 epsilon | MBS-K026 | Human CD3E-coupled Magnetic Beads | |
CD3E & CD3D | MBS-K003 | Human CD3E & CD3D Heterodimer-coupled Magnetic Beads | |
CD47 | MBS-K023 | Human CD47-coupled Magnetic Beads | |
CD47 | MBS-K059 | PE-labeled Human CD47-coupled Magnetic Beads | |
CD47 | MBS-K080 | APC-labeled Human CD47-coupled Magnetic Beads | |
EGF R | MBE-K012 | Human EGFR-coupled Magnetic Beads | |
Her2 | MBS-K006 | Human HER2-coupled Magnetic Beads | |
Mesothelin | MBS-K024 | Human Mesothelin-coupled Magnetic Beads | |
Protein L | MPC-A008 | Protein L-coupled Magnetic Beads (recommended for MPCLIA) | |
HGF R | MBS-K021 | Human HGF R-coupled Magnetic Beads | |
B7-H3 | MBS-K040 | Human B7-H3(4Ig)-coupled Magnetic Beads | |
BCMA | MBS-K004 | Human BCMA-coupled Magnetic Beads | |
CD38 | MBC-K010 | Human CD38-coupled Magnetic Beads | |
ROR1 | MBR-K009 | Human ROR1-coupled Magnetic Beads | |
Siglec-2 | MBS-K052 | Human CD22-coupled Magnetic Beads | |
Siglec-3 | MBC-K008 | Human CD33-coupled Magnetic Beads | |
TROP-2 | MBS-K028 | Human TROP-2-coupled Magnetic Beads | |
Protein A | MPC-A001 | Protein A-coupled Magnetic Beads (recommended for MPCLIA) | |
Protein G | MPC-A002 | Protein G-coupled Magnetic Beads (recommended for MPCLIA) | |
Nectin-4 | MBS-K039 | Human Nectin-4-coupled Magnetic Beads | |
PSMA | MBP-K007 | Human PSMA-coupled Magnetic Beads | |
EGFRvIII | MBS-K020 | Human EGFRvIII-coupled Magnetic Beads | |
FAP | MBS-K077 | Human FAP-coupled Magnetic Beads | |
LRRC15 | MBS-K079 | Human LRRC15-coupled Magnetic Beads | |
His | MPC-A005 | Anti-His Tag Antibody (Mouse IgG1)-coupled Magnetic Beads (recommended for MPCLIA) | |
His | MPC-A007 | Anti-His Tag Antibody, Human IgG1-coupled Magnetic Beads (recommended for MPCLIA) | |
AHG | MPC-A004 | Anti-Human IgG-coupled Magnetic Beads (recommended for MPCLIA) | |
Streptavidin Beads | MPC-A006 | Streptavidin-Magnetic Beads (recommended for MPCLIA) | |
Streptavidin Beads | SMB-B01 | Magnetic Beads™ Streptavidin | |
Mouse IgG | MPC-A003 | Anti-Mouse IgG-coupled Magnetic Beads (recommended for MPCLIA) | |
Anti-Human IgM | MPC-A010 | Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) | |
Anti-FITC | MPC-A009 | Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) |
ACROBiosystems对比多种磁珠,精选出粒径均匀,形状规整,具有超顺磁性的磁珠,得到了最低的非特异性结合。
图2 不同磁珠形态对比(A图为ACROBiosystems精选磁珠)
链霉亲和素(SA)和生物素之间亲和力极强,生物素化蛋白预偶联不可逆。预偶联蛋白磁珠表面包被了饱和浓度的生物素标记抗原,结合能力强,可有效提升捕获效率。
图3 RBD偶联磁珠结合抗体量曲线
图4 RBD偶联磁珠和ACE2蛋白的结合曲线
Protocol
图5 BCMA偶联磁珠和抗BCMA抗体的结合曲线
ACROBiosystems特别设计的预偶联蛋白磁珠为冻干状态,可长期稳定储存,多次冻融不影响结构和性能。重构后即可使用,无需活化,操作简便,可大大提高您的实验效率,节约时间和成本。
图6. ACRO磁珠在不同状态下的电镜图片 (Cat. No. SMB-B01),结果表明磁珠在3次冻融后结构稳定。
图7. 多次冻融后SARS-CoV-2 S RBD蛋白偶联磁珠(货号:MBS-K002)与S1抗体结合曲线与冻融前无显著性差异。
图8. 多次冻融后BCMA蛋白偶联磁珠(货号:MBS-K004)与BCMA抗体结合曲线与冻融前无显著性差异。