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Fc受体蛋白

背景
治疗性抗体的功效不仅取决于其Fab片段结合靶抗原的活性,还取决于其Fc片段与Fc受体的相互作用。Fc片段与FcRn(FCGRT&B2M)受体的亲和力可以预测抗体的半衰期,而Fc片段和FcγRⅢA(CD16a)的结合会影响抗体依赖的细胞介导的细胞毒性作用(Antibody-Dependent Cell-mediated Cytotoxicity)。因此,优化抗体结构,筛选获得和Fc受体亲和力最理想的抗体,是发展治疗性抗体药物的一个重要途径。
ACROBiosystems公司提供一系列重组Fc受体蛋白,包括了常见的突变体,以帮助加速抗体药物的开发。 同时,基于我司丰富的蛋白产品资源和活性分析经验,您只需提供样品,我们即可为您提供优质SPR&BLI亲和力检测服务,且免费提供实验所需所有高质量Fc受体蛋白! 点击快速咨询>>>
热门分子
Fc受体蛋白产品优势

HEK293细胞表达:实现翻译后修饰及蛋白正确折叠

多种属:Human, Mouse, Cynomolgus/Rhesus macaque, Rat, Porcine, Rabbit, Feline, Bovine,可全面应用于不同种属交叉实验

高纯度:SDS-PAGE验证纯度>95%,SEC-MALS验证纯度>90%

低内毒素:<1.0 EU/μg

高亲和力:经SPR&BLI验证,活性有保障,免费提供实验方案

高稳定性:严格质控为高批间一致性保驾护航

提供生物素化定点标记蛋白:采用AvitagTM定点标记的生物素化Fc受体蛋白,标记效率高且标记位点专一明确,方便药物开发及工艺优化过程中基于与链霉亲和素结合的ELISA/SPR/BLI检测

验证数据

产品优势

高纯度

为了满足抗体药研发过程中对用于检测和筛选的蛋白质纯度高的需求,我们的QC团队对大多数重组蛋白都同时进行了SDS-PAGE和SEC-MALS分析。值得注意的是,Fc受体的单体形式反映了其天然状态,应该使用单体纯度尽可能高的Fc受体来验证和抗体的亲和力,以避免因聚集效应而产生的结合活性虚高现象。因此,我们通过SEC-MALS检测建立了严格的单体纯度内部控制标准,只有满足标准的批次才会被放行。

Human CD32a (R167), His Tag (Cat. No. CDA-H5221) MALS images
图1. 经SDS-PAGE验证,Human CD32a (R167), His Tag (Cat. No. CDA-H5221) 纯度高于 95%;经 SEC-MALS 验证,Human CD32a (R167), His Tag (Cat. No. CDA-H5221) 纯度高于 90%,分子量为25-35 kDa。
亲和力经SPR&BLI验证

分子间的相互作用可以通过多种方法检测。传统的ELISA方法不适合检测像CD16和CD32这样的低亲和力受体与抗体的相互作用,而SPR则成为研究Fc受体与抗体亲和力最常用的方法。为了保证我们的Fc受体能够达到预期的性能,我们的QC团队使用Biacore平台对我们大部分产品进行测试。此外,为了满足客户在抗体药研发过程中不同的应用场景,我们同样采用BLI技术进行了亲和力的验证。所有展现在网页上的SPR&BLI数据都免费提供内部实验操作方法供参考

FCA-H52H1-S1
图2. SPR验证,固定在CM5芯片上的Human CD64, His Tag (Cat. No. FCA-H52H1) 可以结合Herceptin ( 赫赛汀单抗 ),亲和常数为4.92 nM ( Biacore 8K )。
申请SPR Protocol
FCM-H5286-S1
图3. SPR验证,将Human FCGRT&B2M Heterodimer Protein (Cat. No. FCM-H5286)通过anti-His抗体固定在CM5芯片上,可以结合Herceptin (赫赛汀单抗),亲和常数为0.261 μM (Biacore T200)。
申请SPR Protocol
FCA-H52H1-B1.jpg
图4. BLI验证,将Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1)固定在HIS1K生物传感器上,可以结合Herceptin (赫赛汀单抗),亲和常数为6.98 nM (ForteBio Octet Red96e)。
申请BLI Protocol
FCN-H52W7-B1
图5. BLI验证,将Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7)通过生物素化的his antibody 固定在SA传感器上,可以结合Herceptin (赫赛汀单抗),亲和常数为0.14 μM (ForteBio Octet Red96e)。
申请BLI Protocol
高批间一致性

我们通过采取严格的质量控制措施,以确保产品的批间一致性。如下图所示,被测样品的批间差异很小。

SPR
图6. SPR验证,固定在CM5芯片上的Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228)可以结合Rituximab (利妥昔单抗),亲和常数为10μM (Biacore T200)。
申请SPR Protocol

用相似度评分软件分析可以看到,不同批次的CD32b相似度非常高,体现了高批间一致性。

RIIB
图7. 不同批次的Human Fc gamma RIIB / CD32b ( Cat. No. CDB-H5228 ) 相似度在90%以上。
产品列表
  • FcRn
  • FcγR
  • FcεR
分子 货号 host 产品描述 结构
FcRn (FCGRT & B2M)FCM-H5286HEK293Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI & MALS verified)
FCM-H8286HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, ultra sensitivity (primary amine labeling) (SPR verified)
FCM-H82W4HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, ,His Tag&Strep II Tag (SPR & BLI & MALS verified)
FCM-H82W7HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
FCN-H52W7HEK293Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI & MALS verified)
FCM-M52W2HEK293Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (MALS & BLI verified)
FCM-M52W8HEK293Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified)
FCM-M82W5HEK293Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & BLI verified)
FCM-M82W6HEK293Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, ,His Tag&Twin-Strep Tag (MALS & BLI-verified)
FCM-C5284HEK293Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & BLI verified)
FCM-C52W9HEK293Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified)
FCM-C82W3HEK293Biotinylated Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
FCM-C82W5HEK293Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,&Strep II Tag (SPR & BLI verified)
FCM-R5287HEK293Rat FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & SPR verified)
FCM-R82W7HEK293Biotinylated Rat FcRn / FCGRT&B2M Heterodimer Protein, ,His Tag&Strep II Tag (MALS & SPR verified)
FCM-P5280HEK293Porcine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & SPR verified)
FCM-R52W5HEK293Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (MALS & SPR verified)
FCN-F82W3HEK293Biotinylated Feline FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
FCN-B82W3HEK293Biotinylated Bovine FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
分子 货号 host 产品描述 结构
Fc gamma RI / CD64FCA-H52H1HEK293Human Fc gamma RI / CD64 Protein, His Tag (MALS verified)
FCA-H82E8HEK293Biotinylated Human Fc gamma RI / CD64 Protein, His, (MALS verified)
CD4-M5227HEK293Mouse Fc gamma RI / CD64 Protein, His Tag (MALS & BLI verified)
CD4-M82E7HEK293Biotinylated Mouse Fc gamma RI / CD64 Protein, His, (MALS & SPR verified)
FCA-C82E8HEK293Biotinylated Cynomolgus Fc gamma RI / CD64 Protein, His, (MALS & BLI verified)
Fc gamma RIIA / CD32aCD1-H5223HEK293Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI & MALS verified)
CDA-H5221HEK293Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (MALS & BLI verified)
CDA-H82E6HEK293Biotinylated Human Fc gamma RIIA / CD32a (H167) Protein, ,His Tag (SPR & BLI & MALS verified)
CDA-H82E7HEK293Biotinylated Human Fc gamma RIIA / CD32a (R167) Protein, ,His Tag (SPR & BLI & MALS verified)
CDA-C52H7HEK293Cynomolgus Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & MALS verified)
CDA-C82E5HEK293Biotinylated Cynomolgus Fc gamma RIIA / CD32a Protein, His, (SPR & BLI & MALS verified)
CDA-R52H5HEK293Rat Fc gamma RIIA / CD32a Protein, His Tag (MALS & SPR verified)
CDA-R52H4HEK293Rhesus macaque Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIB / CD32bCDB-M52H7HEK293Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI & MALS verified)
CDB-M82E8HEK293Biotinylated Mouse Fc gamma RIIB / CD32b Protein, ,His Tag (SPR & BLI & MALS verified)
CDB-C5224HEK293Cynomolgus Fc gamma RIIB / CD32b Protein, His Tag (MALS & SPR verified)
CDB-C82E4HEK293Biotinylated Cynomolgus Fc gamma RIIB / CD32b Protein, His, (SPR & BLI & MALS verified)
Fc gamma RIIB/C / CD32b/cCDB-H5228HEK293Human Fc gamma RIIB/C / CD32b/c Protein, His Tag (SPR & BLI & MALS verified)
CDB-H5298HEK293Human Fc gamma RIIB/C / CD32b/c Protein, SUMO,His Tag (MALS & BLI verified)
CDB-H52S9HEK293Human Fc gamma RIIB/C / CD32b/c Protein, HSA,His Tag (MALS & BLI verified)
CDB-H82E0HEK293Biotinylated Human Fc gamma RIIB/C / CD32b/c Protein, ,His Tag (SPR & BLI & MALS verified)
Fc gamma RIII / CD16CDA-M52H8HEK293Mouse Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI & MALS verified)
FC6-M82E0HEK293Biotinylated Mouse Fc gamma RIII / CD16 Protein, His, (MALS & SPR verified)
FC6-C52H9HEK293Cynomolgus Fc gamma RIII / CD16 Protein, His Tag (MALS & BLI verified)
FC6-C82E0HEK293Biotinylated Cynomolgus Fc gamma RIII / CD16 Protein, His, (MALS & BLI verified)
FC6-R52H6HEK293Rhesus macaque Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIIA / CD16aCD8-H52H4HEK293Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI & MALS verified)
CDA-H5220HEK293Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI & MALS verified)
CDA-H5290HEK293Human Fc gamma RIIIA / CD16a (V176) Protein, SUMO,His Tag (MALS & BLI verified)
CDA-H52H6HEK293Human Fc gamma RIIIA / CD16a (F176, S197P) Protein, His Tag (MALS & SPR verified)
CDA-H52S1HEK293Human Fc gamma RIIIA / CD16a (V176) Protein, HSA,His Tag (MALS & BLI verified)
CDA-H82E8HEK293Biotinylated Human Fc gamma RIIIA / CD16a (F176) Protein, ,His Tag (SPR & BLI & MALS verified)
CDA-H82E9HEK293Biotinylated Human Fc gamma RIIIA / CD16a (V176) Protein, ,His Tag (SPR & BLI verified)
Fc gamma RIIIB / CD16b (NA1)CDB-H5227HEK293Human Fc gamma RIIIB / CD16b (NA1) Protein, His Tag (SPR & BLI & MALS verified)
CDB-H5296HEK293Human Fc gamma RIIIB / CD16b (NA1) Protein, SUMO,His Tag (MALS & BLI-verified)
CDB-H82E4HEK293Biotinylated Human Fc gamma RIIIB / CD16b (NA1) Protein, His, (SPR & BLI & MALS verified)
Fc gamma RIIIB / CD16b (NA2)CDB-H5222HEK293Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI & MALS verified)
CDB-H5294HEK293Human Fc gamma RIIIB / CD16b (NA2) Protein, SUMO,His Tag (MALS & BLI-verified)
CDB-H82EaHEK293Biotinylated Human Fc gamma RIIIB / CD16b (NA2) Protein, His, (SPR & BLI & MALS verified)
Fc gamma RIV / CD16-2FC4-M52H3HEK293Mouse Fc gamma RIV / CD16-2 Protein, His Tag (MALS verified)
FC4-M82E8HEK293Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His, (BLI verified)
分子 货号 host 产品描述 结构
CD23CD3-H5249HEK293Human CD23 / Fc epsilon RII Protein, His Tag (MALS verified)
CD3-H82Q5HEK293Biotinylated Human CD23 / Fc epsilon RII Protein, His, (MALS verified)
Fc epsilon RI alphaFCA-H5228HEK293Human Fc epsilon RI alpha Protein, His Tag (MALS verified)
FCA-H5259HEK293Human Fc epsilon RI alpha Protein, Fc Tag (MALS & BLI verified)

Fc受体相关分子-IgG Fc protein

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