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过表达细胞株
背景介绍
基因过表达是指通过慢病毒、电转等细胞转染方式,提高某特定基因的表达水平,从而实现基因的功能增强(gain of function)。基因过表达作为一种传统的分子生物学技术,被认为是研究基因功能的实验策略之一,至今依然在生物学研究中发挥着重要作用。过表达稳定细胞系(Overexpression Stable Cell Lines)则是基于某一细胞系构建的用于实现某特定基因的持续过表达的产物。过表达细胞株通过将目的基因构建到表达载体上,通过慢病毒侵染等方式导入宿主细胞,整合到染色体上,使目的抗原在宿主细胞膜表面进行长期稳定的表达。
ACROBiosystems百普赛斯基于成熟的细胞构建平台,持续开发高质量的过表达细胞株产品,为新药研发提供一个稳定的细胞水平研发工具!
应用场景

靶点及通路研究

靶点及通路研究

Cross-linking药物评价

Cross-linking药物评价

免疫检查点抗体研究

免疫检查点抗体研究

ADC靶向&CAR-T细胞治疗[1]

ADC靶向&CAR-T细胞治疗

神经科学研究[2]

神经科学研究

糖尿病/肥胖/非酒精性脂肪性肝炎[3]

糖尿病/肥胖/非酒精性脂肪性肝炎

自免疾病药物评价[4]

自免疾病药物评价

产品列表
  • 靶点及通路研究
  • Cross-linking药物评价
  • 免疫检查点抗体研究
  • ADC靶向&CAR-T细胞治疗
  • 神经科学研究
  • 糖尿病/肥胖/非酒精性脂肪性肝炎
  • 自免疾病药物评价
分子 货号 产品描述
ASGR1 CHEK-ATP080 HEK293/Human ASGR1 Stable Cell Line
ASGR1&ASGR2 CHEK-ATP172 HEK293/Human ASGR1&ASGR2 Stable Cell Line
CCR5 CHEK-ATP043 HEK293/Human CCR5 Stable Cell Line
CCR8 CHEK-ATP140 HEK293/Human CCR8 Stable Cell Line
CD20 CHEK-ATP034 HEK293/Human CD20 Stable Cell Line
IDH1(132H)-P2A-mGFP&Luc CHEK-ATP199 HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line
IDH1(132R)-P2A-mGFP&Luc CHEK-ATP200 HEK293/Human IDH1(132R)-P2A-mGFP&Luc Stable Cell Line
NY-ESO-1 CHEK-STP114 NY-ESO-1 specific TCR-HEK293 cell line
SIRP alpha CHEK-ATP051 HEK293/Human SIRP alpha Stable Cell Line
TMPRSS2 CHEK-ATP101 HEK293/Human TMPRSS2-HA-P2A-mGFP Stable Cell Line
分子 货号 产品描述
Fc gamma RI / CD64 SCCHO-ATP062H CHO/Human CD64 Stable Cell Line (High Expression) Development Service
Fc gamma RI / CD64 SCCHO-ATP062L CHO/Human CD64 Stable Cell Line (Low Expression) Development Service
Fc gamma RI / CD64 SCCHO-ATP062M CHO/Human CD64 Stable Cell Line (Medium Expression) Development Service
Fc gamma RIIA / CD32a SCCHO-ATP061H CHO/Human CD32a Stable Cell Line (High Expression) Development Service
Fc gamma RIIA / CD32a SCCHO-ATP061L CHO/Human CD32a Stable Cell Line (Low Expression) Development Service
Fc gamma RIIA / CD32a SCCHO-ATP061M CHO/Human CD32a Stable Cell Line (Medium Expression) Development Service
Fc gamma RIIB / CD32b SCCHO-ATP060H CHO/Human CD32b Stable Cell Line (High Expression) Development Service
Fc gamma RIIB / CD32b SCCHO-ATP060L CHO/Human CD32b Stable Cell Line (Low Expression) Development Service
Fc gamma RIIB / CD32b SCCHO-ATP060M CHO/Human CD32b Stable Cell Line (Medium Expression) Development Service
Fc gamma RIIIA / CD16a SCCHO-ATP059H CHO/Human CD16a (158V) Stable Cell Line (High Expression) Development Service
Fc gamma RIIIA / CD16a SCCHO-ATP059L CHO/Human CD16a (158V) Stable Cell Line (Low Expression) Development Service
Fc gamma RIIIA / CD16a SCCHO-ATP059M CHO/Human CD16a (158V) Stable Cell Line (Medium Expression) Development Service
PD-L1 SCCHO-ATP077H CHO/Human PD-L1 Stable Cell Line (High Expression) Development Service
PD-L1 SCCHO-ATP077L CHO/Human PD-L1 Stable Cell Line (Low Expression) Development Service
PD-L1 SCCHO-ATP077M CHO/Human PD-L1 Stable Cell Line (Medium Expression) Development Service
分子 货号 产品描述
4-1BB CHEK-ATP038 HEK293/Human 4-1BB / TNFRSF9 Stable Cell Line
4-1BB Ligand CHEK-ATP039 HEK293/Human 4-1BB Ligand / TNFSF9 Stable Cell Line
BTLA SCCHO-ATP110 CHO/Human BTLA Stable Cell Line Development Service
CD155 SCRAJ-STT076 Raji/Human CD155 Stable Cell Line Development Service
HVEM CHEK-ATP147 HEK293/Human HVEM Stable Cell Line
HVEM SCRAJ-STF108 Raji/Human HVEM Stable Cell Line Development Service
LIGHT SCCHO-ATP109 CHO/Human LIGHT Stable Cell Line Development Service
LILRB4 CHEK-ATP088 HEK293/Human LILRB4 Stable Cell Line
LILRB4 SCCHO-ATP087 CHO/Human LILRB4 Stable Cell Line Development Service
NKp46 CHEK-ATP153 HEK293/Human NKp46 Stable Cell Line
PD-1 CHEK-ATP143 HEK293/Human PD-1 Stable Cell Line
PD-L1 CHEK-ATP002 HEK293/Human PD-L1, GFP Tag Stable Cell Line
PD-L1 SCRAJ-STT075 Raji/Human PD-L1 Stable Cell Line Development Service
分子 货号 产品描述
B7-H3 (4Ig) SCCHO-ATP169 CHO/Human B7-H3 (4Ig) Stable Cell Line Development Service
B7-H4 CHEK-ATP126 HEK293/Human B7-H4 Stable Cell Line
c-MET&ErbB3 CHEK-ATP217 HEK293/Human c-MET&ErbB3 Stable Cell Line
Cadherin-17 CHEK-ATP173 HEK293/Human Cadherin-17 Stable Cell Line
Cadherin-6 CHEK-ATP127 HEK293/Human Cadherin-6 Stable Cell Line
CD19 CHEK-ATP003 HEK293/Human CD19 Stable Cell Line
CD19 CHEK-ATS056 HEK293/Human Anti-CD19 Stable Cell Line
CD79A & CD79B SCCHO-ATP170 CHO/Human CD79A&CD79B Stable Cell Line Development Service
CD79B SCCHO-ATP171 CHO/Human CD79B Stable Cell Line Development Service
CEACAM-5 CHEK-ATP083 HEK293/Human CEACAM5 Stable Cell Line
CEACAM-5 SCCHO-ATP081 CHO/Human CEACAM5 Stable Cell Line Development Service
Claudin-1 CHEK-ATP124 HEK293/Human Claudin-1 Stable Cell Line
Claudin-18.2 CHEK-ATP033 HEK293/Human Claudin-18.2 Stable Cell Line
Claudin-6 CHEK-ATP138 HEK293/Human Claudin-6 Stable Cell Line
Claudin-9 CHEK-ATP139 HEK293/Human Claudin-9 Stable Cell Line
DLL3 CHEK-ATP090 HEK293/Human DLL3 Stable Cell Line
DLL3 SCCHO-ATP111 CHO/Human DLL3 Stable Cell Line Development Service
EGF R CHEK-ATP148 HEK293/Human EGF R Stable Cell Line
ENPP3 CHEK-ATP122 HEK293/Human ENPP3 Stable Cell Line
EpCAM CHEK-ATP175 HEK293/Human EpCAM Stable Cell Line
ErbB3 CHEK-ATP149 HEK293/Human ErbB3 Stable Cell Line
FAP CHEK-ATP184 HEK293/Human FAP Stable Cell Line
FOLR1 CHEK-ATP091 HEK293/Human FOLR1 Stable Cell Line
Glypican 3 CHEK-ATP092 HEK293/Human Glypican-3 (GPC3) Stable Cell Line
Glypican 3 CHEK-ATP177 HEK293/Cynomolgus Glypican-3 (GPC3) Stable Cell Line
Glypican 3 SCCHO-ATP112 CHO/Human Glypican-3 (GPC3) Stable Cell Line Development Service
Glypican-3 SCCHO-ATP179 CHO/Cynomolgus Glypican-3 (GPC3) Stable Cell Line Development Service
GPC3 CHEK-ATP212 HEK293/Human GPC3 ΔHS Stable Cell Line
GPRC5D CCHO-STP078 CHO/Human GPRC5D Stable Cell Line
GPRC5D CHEK-STP042 HEK293/Human GPRC5D Stable Cell Line
GUCY2C CHEK-ATP182 HEK293/Human GUCY2C Stable Cell Line
Her2 CHEK-ATP150 HEK293/Human ErbB2 Stable Cell Line
HGF R CHEK-ATP146 HEK293/Human c-MET Stable Cell Line
HGF R SCCHO-ATP141 CHO/Human c-MET Stable Cell Line Development Service
Integrin alpha V beta 6 CHEK-ATP125 HEK293/Human Integrin alpha V beta 6 Stable Cell Line
LRRC15 CHEK-ATP123 HEK293/Human LRRC15 Stable Cell Line
LY6G6D CHEK-ATP137 HEK293/Human LY6G6D Stable Cell Line
MCAM CHEK-ATP195 HEK293/Human MCAM Stable Cell Line
Mesothelin CHEK-ATP119 HEK293/Human Mesothelin Stable Cell Line
Mesothelin SCCHO-ATP120 CHO/Human Mesothelin Stable Cell Line Development Service
NAPI-IIb CHEK-ATP116 HEK293/Human NAPI-IIb Stable Cell Line
Nectin-4 CHEK-ATP035 HEK293/Human Nectin-4 Stable Cell Line
PSMA CHEK-ATP185 HEK293/Human PSMA Stable Cell Line
PTK7 CHEK-ATP186 HEK293/Human PTK7 Stable Cell Line
ROR1 CHEK-ATP084 HEK293/Human ROR1 Stable Cell Line
ROR1 SCCHO-ATP083 CHO/Human ROR1 Stable Cell Line Development Service
SEZ6 CHEK-ATP183 HEK293/Human SEZ6 Stable Cell Line
SSTR2 CHEK-ATP213 HEK293/Human SSTR2 Stable Cell Line
STEAP1 CHEK-ATP154 HEK293/Human STEAP1 Stable Cell Line
STEAP1 SCCHO-ATP121 CHO/Human STEAP1 Stable Cell Line Development Service
TPBG CHEK-ATP176 HEK293/Human TPBG Stable Cell Line
Transferrin CHEK-ATP115 HEK293/Human Transferrin Stable Cell Line
Transferrin R CHEK-ATP089 HEK293/Human Transferrin R Stable Cell Line
TROP-2 CHEK-ATP036 HEK293/Human TROP-2 Stable Cell Line
uPAR CHEK-ATP151 HEK293/Human uPAR Stable Cell Line
uPAR SCCHO-ATP152 CHO/Human uPAR Stable Cell Line Development Service
分子 货号 产品描述
Alpha-Synuclein CHEK-ATP085 HEK293/Human Alpha-synuclein (GFP) Stable Cell Line
APP CHEK-ATP081 HEK293/Human APP (GFP) Stable Cell Line
LDL R CHEK-ATP158 HEK293/Human LDL R Stable Cell Line
LILRB3 CHEK-ATP159 HEK293/Human LILRB3 Stable Cell Line
NGFR CHEK-ATP157 HEK293/Human NGFR Stable Cell Line
RAGE CHEK-ATP156 HEK293/Human RAGE Stable Cell Line
Sortilin CHEK-ATP155 HEK293/Human SORT1 Stable Cell Line
Tau CHEK-ATP087 HEK293/Human Tau-K18 (GFP) Stable Cell Line
TrkA CHEK-ATP192 HEK293/Human TrkA Stable Cell Line
TrkB CHEK-ATP082 HEK293/Human TrkB Stable Cell Line
TrkC CHEK-ATP189 HEK293/Human TrkC Stable Cell Line
分子 货号 产品描述
GCGR CHEK-ATP209 HEK293/Human GCGR Stable Cell Line (High Expression)
GCGR CHEK-ATP210 HEK293/Human GCGR Stable Cell Line (Medium Expression)
GCGR CHEK-ATP211 HEK293/Human GCGR Stable Cell Line (Low Expression)
GIPR CHEK-ATP206 HEK293/Human GIPR Stable Cell Line (High Expression)
GIPR CHEK-ATP207 HEK293/Human GIPR Stable Cell Line (Medium Expression)
GIPR CHEK-ATP208 HEK293/Human GIPR Stable Cell Line (Low Expression)
GLP-1R&GIPR CHEK-ATP205 HEK293/Human GLP-1R&GIPR Stable Cell Line
GLP1R CHEK-ATP160 HEK293/Human GLP-1R Stable Cell Line (High Expression)
GLP1R CHEK-ATP161 HEK293/Human GLP-1R Stable Cell Line (Medium Expression)
GLP1R CHEK-ATP162 HEK293/Human GLP-1R Stable Cell Line (Low Expression)
GPR75 CHEK-ATP174 HEK293/Human GPR75 Stable Cell Line
分子 货号 产品描述
CD40 Ligand CHEK-ATP041 HEK293/Human CD40 Ligand / TNFSF5 Stable Cell Line
FcRn SCCHO-ATP193 CHO/Mouse FCGRT-P2A-mGFP&B2M Cell Line Development Service
FcRn SCMDC-ATP196 MDCK/Mouse FCGRT-P2A-mGFP&B2M Cell Line Development Service
FcRn (FCGRT & B2M) CHEK-ATP079 HEK293/Human FcRn (FCGRT & B2M) Stable Cell Line
FcRn (FCGRT & B2M) CHEK-ATP132 HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line
IGF-I R CNIH-ATP102 NIH-3T3/Human IGF-1 R Stable Cell Line Development Service
MRGPRX2 CHEK-ATP214 HEK293/Human MRGPRX2 Stable Cell Line
MRGPRX2 SCCHO-ATP215 CHO/Human MRGPRX2 Stable Cell Line Development Service
OX40 CHEK-ATP053 HEK293/Human OX40 / TNFRSF4 / CD134 Stable Cell Line
OX40 Ligand CHEK-ATP054 HEK293/Human OX40 Ligand / TNFSF4 Stable Cell Line
TL1A CHEK-ATP142 HEK293/Human TL1A Stable Cell Line
TL1A CHEK-ATP198 HEK293/Membrane-Bound human TL1A Stable Cell Line
TL1A SCRAJ-STT204 Raji/Membrane-Bound Human TL1A Stable Cell Line Development Service
TSHR CHEK-ATP086 HEK293/Human TSHR Stable Cell Line
TSHR SCCHO-ATP085 CHO/Human TSHR Stable Cell Line Development Service
验证数据
Receptor Assay
CAR-T靶细胞开发

Expression analysis of human CD64 on CHO/Human CD64 Stable Cell Line by FACS. Cell surface staining using PE-labeled anti-human CD64 antibody was performed on CHO/Human CD64 Stable Cell Line with different expression levels: CHO/Human CD64 Stable Cell Line (Low Expression) (Cat. No. SCCHO-ATP062L); CHO/Human CD64 Stable Cell Line (Medium Expression) (Cat. No. SCCHO-ATP062M); CHO/Human CD64 Stable Cell Line (High Expression) (Cat. No. SCCHO-ATP062H).

Application
单抗活性筛选

Bioactivity analysis of anti-human 4-1BB antibody through CHO/Human CD32b Stable Cell Line (Medium Expression) (Cat. No. SCCHO-ATP060M) crosslinking to test whether in a CD32b-dependent manner to strengthen the agonistic activity. The EC50 of anti-human 4-1BB antibody is approximately 0.18 μg/mL through CHO/Human CD32b Stable Cell Line (Medium Expression) crosslinking.

单抗活性筛选

Blocking activity of anti-human PD-1 antibody (FOLD). This Raji/Human PD-L1 Stable Cell Line (Cat. No. SCRAJ-STT075) was incubated with serial dilutions of antibodies in the presence of reporter cells expressing human PD-1. The max induction fold was approximately 22.47.

更多产品及服务

参考文献

[1]. Yu B, Jiang T, Liu D. BCMA-targeted immunotherapy for multiple myeloma. J Hematol Oncol. 2020;13(1):125. Published 2020 Sep 17. doi:10.1186/s13045-020-00962-7

[2]. Raimondo L, Oliveira ĹAF, Heij J, et al. Advances in resting state fMRI acquisitions for functional connectomics. Neuroimage. 2021;243:118503. doi:10.1016/j.neuroimage.2021.118503

[3]. Boland ML, Laker RC, Mather K, et al. Resolution of NASH and hepatic fibrosis by the GLP-1R/GcgR dual-agonist Cotadutide via modulating mitochondrial function and lipogenesis. Nat Metab. 2020;2(5):413-431. doi:10.1038/s42255-020-0209-6

[4]. Xue C, Yao Q, Gu X, et al. Evolving cognition of the JAK-STAT signaling pathway: autoimmune disorders and cancer. Signal Transduct Target Ther. 2023;8(1):204. Published 2023 May 19. doi:10.1038/s41392-023-01468-7

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